验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HeLa cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16490-1-AP targets RPS12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, drosophila |
| 免疫原 | RPS12 fusion protein Ag9615 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S12 |
| 别名 | Small ribosomal subunit protein eS12, ribosomal protein S12, 40S ribosomal protein S12 |
| 计算分子量 | 132 aa, 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC017321 |
| 基因名称 | RPS12 |
| Gene ID (NCBI) | 6206 |
| RRID | AB_2146233 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25398 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.This antibody specifically recognizes the 15kd RPS12 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS12 antibody 16490-1-AP | Download protocol |
| IHC protocol for RPS12 antibody 16490-1-AP | Download protocol |
| IF protocol for RPS12 antibody 16490-1-AP | Download protocol |
| IP protocol for RPS12 antibody 16490-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity | ||
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Dev Cell Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions | ||
Dev Cell Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure. | ||
Sci Signal Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas |
