验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse oterus tissue, rat oterus tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RPS13 Ready-To-Use IHC Kit. RPS13 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
16680-1-AP targets RPS13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | RPS13 fusion protein Ag10008 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribosomal protein S13 |
| Synonyms | Small ribosomal subunit protein uS15, ribosomal protein S13, 40S ribosomal protein S13 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 14 kDa, 17 kDa |
| GenBank Accession Number | BC006772 |
| Gene Symbol | RPS13 |
| Gene ID (NCBI) | 6207 |
| RRID | AB_2182500 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62277 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS13, also named as 40S ribosomal protein S13, belongs to the ribosomal protein S15P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. RPS13 is located in the cytoplasm. It is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS13 antibody 16680-1-AP | Download protocol |
| IHC protocol for RPS13 antibody 16680-1-AP | Download protocol |
| IF protocol for RPS13 antibody 16680-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cells Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells. | ||
Cell An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes |
