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RPS15A Polyclonal antibody

RPS15A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14942-1-AP

Print datasheet

Synonyms

40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a

验证数据展示

  • IHC staining of human kidney using 14942-1-AP

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14942-1-AP

    WB analysis using 14942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14942-1-AP (RPS15A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14942-1-AP

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14942-1-AP targets RPS15A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen RPS15A fusion protein Ag6655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S15a
Synonyms 40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a
Calculated Molecular Weight 15 kDa
Observed Molecular Weight13-15 kDa
GenBank Accession NumberBC001697
Gene Symbol RPS15A
Gene ID (NCBI) 6210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS15A is a component of the small ribosomal subunit, it is required for proper erythropoiesis.

实验方案

Product Specific Protocols
WB protocol for RPS15A antibody 14942-1-APDownload protocol
IHC protocol for RPS15A antibody 14942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Med

MCM8 promotes gastric cancer progression through RPS15A and predicts poor prognosis

Authors - Lixian Ding
View Article