验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
15603-1-AP targets RPS16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | RPS16 fusion protein Ag7986 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S16 |
| 别名 | Small ribosomal subunit protein uS9, ribosomal protein S16, 40S ribosomal protein S16 |
| 计算分子量 | 16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC004324 |
| 基因名称 | RPS16 |
| Gene ID (NCBI) | 6217 |
| RRID | AB_2180168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62249 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS16, the 40S ribosmal protein S16, belongs to the ribosomal protein S9P family. It is located in the cytoplasm and can be acetylated. RPS16 is extensively involved in rRNA processing, translational elongation, initiation and termination via its RNA binding activity. Catalog#15603-1-AP is a rabbit polyclonal antibody raised against full-length RPS16 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS16 antibody 15603-1-AP | Download protocol |
| IHC protocol for RPS16 antibody 15603-1-AP | Download protocol |
| IF protocol for RPS16 antibody 15603-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas | ||
Proteomics Clin Appl New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells. | ||
Int J Biol Markers Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation |
