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RPS17 Polyclonal antibody

RPS17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16267-1-AP

Print datasheet

Synonyms

RPS17L2, RPS17L1, RPS17L, ribosomal protein S17, DBA4

验证数据展示

  • WB analysis using 16267-1-AP

    WB analysis using 16267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16267-1-AP (RPS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 16267-1-AP

    IHC staining of mouse cerebellum using 16267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 16267-1-AP

    IHC staining of mouse cerebellum using 16267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 16267-1-AP

    IF Staining of U2OS using 16267-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (16267-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, PC-3 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16267-1-AP targets RPS17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RPS17 fusion protein Ag9334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S17
Synonyms RPS17L2, RPS17L1, RPS17L, ribosomal protein S17, DBA4
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC009407
Gene Symbol RPS17
Gene ID (NCBI) 6218
RRIDAB_3085483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08708
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS17 antibody 16267-1-APDownload protocol
IHC protocol for RPS17 antibody 16267-1-APDownload protocol
IF protocol for RPS17 antibody 16267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols