RPS19 Polyclonal antibody

RPS19 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15085-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

40S ribosomal protein S19, DBA, ribosomal protein S19, RPS19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCaco-2 cells, human testis tissue, HEK-293 cells, rat colon tissue, mouse testis tissue, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15085-1-AP targets RPS19 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RPS19 fusion protein Ag7164 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S19
别名 40S ribosomal protein S19, DBA, ribosomal protein S19, RPS19
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000023
基因名称 RPS19
Gene ID (NCBI) 6223
RRIDAB_2180202
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39019
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes. The RPS19 protein is a component of the 40S ribosomal subunit [PMID: 17726054]. Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Among these genes, ribosomal protein S19 (RPS19) is mutated most frequently [PMID:21989989]. It also a p53-related ribosomal protein that possibly involves cellular apoptosis through the BAX/p53 pathway [PMID:22272377].

实验方案

Product Specific Protocols
WB protocol for RPS19 antibody 15085-1-APDownload protocol
IHC protocol for RPS19 antibody 15085-1-APDownload protocol
IP protocol for RPS19 antibody 15085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs

Authors - James C Murphy
WB,IF

Development

Msl3 promotes germline stem cell differentiation in female Drosophila.

Authors - Alicia McCarthy
mouseIHC

J Proteome Res

Human SOD1-G93A specific distribution evidenced in murine brain of a transgenic model for Amyotrophic Lateral Sclerosis by MALDI Imaging Mass Spectrometry.

Authors - Elena Acquadro
humanWB

Sci Rep

RNA G-quadruplex structures control ribosomal protein production

Authors - DhavalVarshney
humanWB

BBA Clin

Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay.

Authors - Joy Armistead
mouseWB

Adv Sci (Weinh)

Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.

Authors - Zhiyuan Lu
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