验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy RPS20 Ready-To-Use IHC Kit. RPS20 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
15692-1-AP targets RPS20 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPS20 fusion protein Ag8257 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S20 |
| 别名 | 40S ribosomal protein S20, ribosomal protein S20, RPS20 |
| 计算分子量 | 13 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC007507 |
| 基因名称 | RPS20 |
| Gene ID (NCBI) | 6224 |
| RRID | AB_10596626 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P60866 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS20 antibody 15692-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity | ||
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Oncol Rep Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma
| ||
J Pineal Res Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin | ||
bioRxiv RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages |
