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  • Featured Product
  • KD/KO Validated

RPS26 Polyclonal antibody

RPS26 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14909-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein eS26, ribosomal protein S26, 40S ribosomal protein S26

验证数据展示

  • WB analysis of mouse ovary using 14909-1-AP

    WB analysis of mouse ovary using 14909-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14909-1-AP (RPS26 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14909-1-AP

    IF Staining of MCF-7 using 14909-1-AP

    Immunofluorescent analysis of MCF-7 cells, using RPS26 antibody 14909-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse lung using 14909-1-AP

    IP experiment of mouse lung using 14909-1-AP

    IP result of anti-RPS26 (IP:14909-1-AP, 4ug; Detection:14909-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • IHC staining of human breast cancer using 14909-1-AP

    IHC staining of human breast cancer using 14909-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 14909-1-AP

    IHC staining of human kidney using 14909-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse ovary tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14909-1-AP targets RPS26 in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, PLA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPS26 fusion protein Ag6706 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S26
Synonyms Small ribosomal subunit protein eS26, ribosomal protein S26, 40S ribosomal protein S26
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC002604
Gene Symbol RPS26
Gene ID (NCBI) 6231
RRIDAB_2180361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.

实验方案

Product Specific Protocols
WB protocol for RPS26 antibody 14909-1-APDownload protocol
IHC protocol for RPS26 antibody 14909-1-APDownload protocol
IF protocol for RPS26 antibody 14909-1-APDownload protocol
IP protocol for RPS26 antibody 14909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
View Article

Nat Commun

Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.

Authors - Yanqin Yu
View Article
humanWB

Nat Commun

Genome-wide search for exonic variants affecting translational efficiency.

Authors - Li Quan Q
View Article
humanIHC, WB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
View Article
humanWB, PLA

Elife

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Authors - Max Koppers
View Article
humanWB,IP

Oncogene

The ribosomal protein S26 regulates p53 activity in response to DNA damage.

Authors - Cui D D
  • KD Validated
View Article