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RPS29 Polyclonal antibody

RPS29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17374-1-AP

Print datasheet

Synonyms

40S ribosomal protein S29, ribosomal protein S29, RPS29

验证数据展示

  • WB analysis using 17374-1-AP

    WB analysis using 17374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17374-1-AP (RPS29 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17374-1-AP

    IHC staining of mouse brain using 17374-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17374-1-AP (RPS29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17374-1-AP

    IHC staining of mouse brain using 17374-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17374-1-AP (RPS29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17374-1-AP targets RPS29 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RPS29 fusion protein Ag11179 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S29
Synonyms 40S ribosomal protein S29, ribosomal protein S29, RPS29
Calculated Molecular Weight 56 aa, 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC035313
Gene Symbol RPS29
Gene ID (NCBI) 6235
RRIDAB_2180751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPS29 antibody 17374-1-APDownload protocol
IHC protocol for RPS29 antibody 17374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols