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RPS3A Polyclonal antibody

RPS3A Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 14123-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, FC (Intra), IP, ELISA and More (1)

ribosomal protein S3A, MFTL, Fte-1, FTE1, Fte 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A431 cells, Jurkat cells, A549 cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPS3A Ready-To-Use IHC Kit. RPS3A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14123-1-AP targets RPS3A in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, xenopus
免疫原 RPS3A fusion protein Ag5273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S3A
别名 ribosomal protein S3A, MFTL, Fte-1, FTE1, Fte 1
计算分子量 30 kDa
观测分子量 35 kDa
GenBank蛋白编号BC066926
基因名称 RPS3A
Gene ID (NCBI) 6189
RRIDAB_2253921
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors

实验方案

Product Specific Protocols
WB protocol for RPS3A antibody 14123-1-APDownload protocol
IHC protocol for RPS3A antibody 14123-1-APDownload protocol
IF protocol for RPS3A antibody 14123-1-APDownload protocol
IP protocol for RPS3A antibody 14123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
XenopusWB

Neuron

Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.

Authors - Jean-Michel Cioni
humanWB

Mol Cell Proteomics

Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.

Authors - Qi Tong-Gang TG
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li
human,mouseWB,IHC,IF

Cell Discov

RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases.

Authors - Yan Tang
  • KD Validated
IF

Cell Rep

On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.

Authors - Christine E. Holt
XenopusIF

Cell

Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.

Authors - Jean-Michel Cioni
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