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RPS4Y1 Polyclonal antibody

RPS4Y1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17296-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

验证数据展示

  • WB analysis of DU 145 using 17296-1-AP

    WB analysis of DU 145 using 17296-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17296-1-AP

    IF Staining of HepG2 using 17296-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPS4Y1 antibody 17296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human appendicitis using 17296-1-AP

    IHC staining of human appendicitis using 17296-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inDU 145 cells, A2780 cells, HeLa cells, mouse ovary tissue, mouse testis tissue, SKOV-3 cells
Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

17296-1-AP targets RPS4Y1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPS4Y1 fusion protein Ag11176 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S4, Y-linked 1
Synonyms Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4
Calculated Molecular Weight 263 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC010286
Gene Symbol RPS4Y1
Gene ID (NCBI) 6192
RRIDAB_2180889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22090
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.

实验方案

Product Specific Protocols
WB protocol for RPS4Y1 antibody 17296-1-APDownload protocol
IHC protocol for RPS4Y1 antibody 17296-1-APDownload protocol
IF protocol for RPS4Y1 antibody 17296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

iScience

Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration

Authors - Fangbo Liu
View Article