S6 Ribosomal protein Polyclonal antibody

S6 Ribosomal protein Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14823-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IP, IHC, ELISA and More (2)

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, mouse kidney tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14823-1-AP targets S6 Ribosomal protein in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF, IP, RIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, zebrafish
免疫原 S6 Ribosomal protein fusion protein Ag6599 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6
别名 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein
计算分子量 29 kDa
观测分子量 29-32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
RRIDAB_2181025
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

实验方案

Product Specific Protocols
WB protocol for S6 Ribosomal protein antibody 14823-1-APDownload protocol
IHC protocol for S6 Ribosomal protein antibody 14823-1-APDownload protocol
IP protocol for S6 Ribosomal protein antibody 14823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Authors - Xiang Xu
humanWB

Nat Commun

Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

Authors - Evangelia Lekka
humanWB

Nat Commun

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Authors - Matthias M Zimmer
humanWB

Mol Ther

RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.

Authors - Jiexin Li
mouseWB

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis

Authors -
humanWB

Oncotarget

Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer.

Authors - Dong Wang
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