S6 Ribosomal protein Polyclonal antibody
S6 Ribosomal protein Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, MCF-7 cells, mouse kidney tissue |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14823-1-AP targets S6 Ribosomal protein in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IF, IP, RIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | S6 Ribosomal protein fusion protein Ag6599 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribosomal protein S6 |
Synonyms | 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein |
Calculated Molecular Weight | 29 kDa |
Observed Molecular Weight | 29-32 kDa |
GenBank Accession Number | BC000524 |
Gene Symbol | RPS6 |
Gene ID (NCBI) | 6194 |
RRID | AB_2181025 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62753 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for S6 Ribosomal protein antibody 14823-1-AP | Download protocol |
IHC protocol for S6 Ribosomal protein antibody 14823-1-AP | Download protocol |
IP protocol for S6 Ribosomal protein antibody 14823-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease | ||
Nat Commun The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting. | ||
Mol Ther RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin. | ||
Development MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis | ||
Oncotarget Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer. |