验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HeLa cells, A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29224-1-AP targets RPS6KA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
别名 | MAPKAPK 1a, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1 |
计算分子量 | 735 aa, 83 kDa |
观测分子量 | 75-95 kDa |
GenBank蛋白编号 | BC014966 |
基因名称 | RPS6KA1 |
Gene ID (NCBI) | 6195 |
RRID | AB_3669677 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q15418 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation.
实验方案
Product Specific Protocols | |
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WB protocol for RPS6KA1 antibody 29224-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |