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S6K2 Polyclonal antibody

S6K2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15268-1-AP

Print datasheet

Synonyms

p70 S6 kinase beta, RPS6KB2, p70 ribosomal S6 kinase beta, P70 beta 2, P70 beta 1

验证数据展示

  • IHC staining of human kidney using 15268-1-AP

    IHC staining of human kidney using 15268-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15268-1-AP

    IHC staining of human kidney using 15268-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 15268-1-AP

    WB analysis of MCF-7 using 15268-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 15268-1-AP

    IP experiment of MCF-7 using 15268-1-AP

    IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

  • IF Staining of MCF-7 using 15268-1-AP

    IF Staining of MCF-7 using 15268-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15268-1-AP targets S6K2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen S6K2 fusion protein Ag7371 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 2
Synonyms p70 S6 kinase beta, RPS6KB2, p70 ribosomal S6 kinase beta, P70 beta 2, P70 beta 1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC000094
Gene Symbol RPS6KB2
Gene ID (NCBI) 6199
RRIDAB_2182250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).

实验方案

Product Specific Protocols
WB protocol for S6K2 antibody 15268-1-APDownload protocol
IHC protocol for S6K2 antibody 15268-1-APDownload protocol
IF protocol for S6K2 antibody 15268-1-APDownload protocol
IP protocol for S6K2 antibody 15268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

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IHC

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Authors - Linbi Xie
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