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验证数据展示

  • IHC staining of human kidney using 15268-1-AP

    IHC staining of human kidney using 15268-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15268-1-AP

    IHC staining of human kidney using 15268-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 15268-1-AP

    WB analysis of MCF-7 using 15268-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 15268-1-AP

    IP experiment of MCF-7 using 15268-1-AP

    IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

  • IF Staining of MCF-7 using 15268-1-AP

    IF Staining of MCF-7 using 15268-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

S6K2 Polyclonal antibody

S6K2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15268-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

p70 S6 kinase beta, RPS6KB2, p70 ribosomal S6 kinase beta, P70 beta 2, P70 beta 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15268-1-AP targets S6K2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 S6K2 fusion protein Ag7371 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 70kDa, polypeptide 2
别名 p70 S6 kinase beta, RPS6KB2, p70 ribosomal S6 kinase beta, P70 beta 2, P70 beta 1
计算分子量 53 kDa
观测分子量 54 kDa
GenBank蛋白编号BC000094
基因名称 RPS6KB2
Gene ID (NCBI) 6199
RRIDAB_2182250
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).

实验方案

Product Specific Protocols
WB protocol for S6K2 antibody 15268-1-APDownload protocol
IHC protocol for S6K2 antibody 15268-1-APDownload protocol
IF protocol for S6K2 antibody 15268-1-APDownload protocol
IP protocol for S6K2 antibody 15268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
View Article
IHC

J Ethnopharmacol

Exploring the potential mechanism of Heng-Gu-Gu-Shang-Yu-He-Ji therapy for osteoporosis based on network pharmacology and transcriptomics

Authors - Linbi Xie
View Article