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验证数据展示

  • WB analysis using 25578-1-AP

    WB analysis using 25578-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25578-1-AP (RPUSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25578-1-AP

    IP experiment of HeLa using 25578-1-AP

    IP result of anti-RPUSD1 (IP:25578-1-AP, 4ug; Detection:25578-1-AP 1:500) with HeLa cells lysate 1760 ug.

  • IF Staining of HeLa using 25578-1-AP

    IF Staining of HeLa using 25578-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPUSD1 antibody (25578-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

RPUSD1 Polyclonal antibody

RPUSD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25578-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

RLUCL, C16orf40

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25578-1-AP targets RPUSD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 RPUSD1 fusion protein Ag22314 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA pseudouridylate synthase domain containing 1
别名 RLUCL, C16orf40
计算分子量 312 aa, 35 kDa
观测分子量34~35 kDa
GenBank蛋白编号BC011783
基因名称 RPUSD1
Gene ID (NCBI) 113000
RRIDAB_3669487
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPUSD1 (RNA pseudouridylate synthase domain-containing protein 1) also known as RLUCL (Ribosomal large subunit pseudouridine synthase C-like protein), was significantly upregulated in HCC and expressed at higher levels in advanced stage or poorly differentiated HCC tissues (PMID: 36437949).

实验方案

Product Specific Protocols
WB protocol for RPUSD1 antibody 25578-1-APDownload protocol
IF protocol for RPUSD1 antibody 25578-1-APDownload protocol
IP protocol for RPUSD1 antibody 25578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols