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  • Featured Product
  • KD/KO Validated

RRAD Polyclonal antibody

RRAD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27763-1-AP

Print datasheet

Synonyms

Ras associated with diabetes, RAD1, RAD, GTP-binding protein RAD, GTP binding protein RAD

验证数据展示

  • WB analysis using 27763-1-AP

    WB analysis using 27763-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27763-1-AP (RRAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of SKOV-3 using 27763-1-AP

    WB analysis of SKOV-3 using 27763-1-AP

    WB result of RRAD antibody (27763-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAD transfected SKOV-3 cells.

经过测试的应用

Positive WB detected inmouse heart tissue, SKOV-3 cells, rat heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27763-1-AP targets RRAD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RRAD fusion protein Ag26869 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras-related associated with diabetes
Synonyms Ras associated with diabetes, RAD1, RAD, GTP-binding protein RAD, GTP binding protein RAD
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 29-34 kDa
GenBank Accession NumberBC057815
Gene Symbol RRAD
Gene ID (NCBI) 6236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RRAD is also named as RAD1. RRAD, a member of the Ras-like small GTPase family, was initially identified as a gene associated with Type II diabetes since it was found to be overexpressed in some Type II diabetic patients (PMID: 8248782). RRAD overexpression reduced insulin-stimulated glucose uptake in cultured muscle and adipocytes cells (PMID: 8798502). RRAD was found to be frequently down-regulated in different types of human cancers, including lung cancer, breast cancer, and nasopharyngeal carcinoma, etc, due to the hypermethylation of its promoter (PMID: 17195088).

实验方案

Product Specific Protocols
WB protocol for RRAD antibody 27763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols