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验证数据展示

  • WB analysis using 15315-1-AP

    WB analysis using 15315-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15315-1-AP (RRAGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 15315-1-AP

    IP experiment of NIH/3T3 using 15315-1-AP

    IP result of anti-RRAGD (IP:15315-1-AP, 4ug; Detection:15315-1-AP 1:500) with NIH/3T3 cells lysate 1120 ug.

  • IF Staining of HepG2 using 15315-1-AP

    IF Staining of HepG2 using 15315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRAGD antibody (15315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

RRAGD Polyclonal antibody

RRAGD Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15315-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Ras-related GTP-binding protein D, Ras related GTP binding D, RagD, Rag D, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inNIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15315-1-AP targets RRAGD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RRAGD fusion protein Ag7557 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-related GTP binding D
别名 Ras-related GTP-binding protein D, Ras related GTP binding D, RagD, Rag D, EC:3.6.5.-
计算分子量 46 kDa
观测分子量46-50 kDa
GenBank蛋白编号BC003088
基因名称 RRAGD
Gene ID (NCBI) 58528
RRIDAB_3085458
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQL2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.

实验方案

Product Specific Protocols
WB protocol for RRAGD antibody 15315-1-APDownload protocol
IF protocol for RRAGD antibody 15315-1-APDownload protocol
IP protocol for RRAGD antibody 15315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols