验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, PC-3 cells, HT-29 cells |
Positive IP detected in | HT-29 cells |
Positive IHC detected in | human renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IHC | See 1 publications below |
产品信息
27457-1-AP targets RRAS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | RRAS fusion protein Ag26538 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | related RAS viral (r-ras) oncogene homolog |
别名 | p23, Ras related protein R Ras, RRAS |
计算分子量 | 218 aa, 23 kDa |
观测分子量 | 23 kDa |
GenBank蛋白编号 | BC016318 |
基因名称 | RRAS |
Gene ID (NCBI) | 6237 |
RRID | AB_2880876 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P10301 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RRAS antibody 27457-1-AP | Download protocol |
IHC protocol for RRAS antibody 27457-1-AP | Download protocol |
IP protocol for RRAS antibody 27457-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma | ||
Biomaterials Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy. | ||
Transl Cancer Res Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses. | ||
Hepatology Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis | ||
Cancer Lett Integrated multi-omics analyses of oral squamous cell carcinoma reveal precision patient stratification and personalized treatment strategies |