RRAS Polyclonal antibody

RRAS Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27457-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

p23, Ras related protein R Ras, RRAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, PC-3 cells, HT-29 cells
Positive IP detected inHT-29 cells
Positive IHC detected inhuman renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27457-1-AP targets RRAS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 RRAS fusion protein Ag26538 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 related RAS viral (r-ras) oncogene homolog
别名 p23, Ras related protein R Ras, RRAS
计算分子量 218 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC016318
基因名称 RRAS
Gene ID (NCBI) 6237
RRIDAB_2880876
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10301
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRAS antibody 27457-1-APDownload protocol
IHC protocol for RRAS antibody 27457-1-APDownload protocol
IP protocol for RRAS antibody 27457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Authors - Perla Pucci
human,mouseWB

Biomaterials

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Authors - Yuanyuan Liu
humanWB

Transl Cancer Res

Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses.

Authors - Ruowen Xiao
humanWB

Hepatology

Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis

Authors - Meilin Ma
IHC

Cancer Lett

Integrated multi-omics analyses of oral squamous cell carcinoma reveal precision patient stratification and personalized treatment strategies

Authors - Chi-Sheng Wu
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