RRAS Polyclonal antibody

RRAS Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27457-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

p23, Ras related protein R Ras, RRAS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, PC-3 cells, HT-29 cells
Positive IP detected inHT-29 cells
Positive IHC detected inhuman renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27457-1-AP targets RRAS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RRAS fusion protein Ag26538 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name related RAS viral (r-ras) oncogene homolog
Synonyms p23, Ras related protein R Ras, RRAS
Calculated Molecular Weight 218 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC016318
Gene Symbol RRAS
Gene ID (NCBI) 6237
RRIDAB_2880876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10301
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRAS antibody 27457-1-APDownload protocol
IHC protocol for RRAS antibody 27457-1-APDownload protocol
IP protocol for RRAS antibody 27457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Authors - Perla Pucci
human,mouseWB

Biomaterials

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Authors - Yuanyuan Liu
humanWB

Transl Cancer Res

Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses.

Authors - Ruowen Xiao
humanWB

Hepatology

Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis

Authors - Meilin Ma
IHC

Cancer Lett

Integrated multi-omics analyses of oral squamous cell carcinoma reveal precision patient stratification and personalized treatment strategies

Authors - Chi-Sheng Wu
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