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验证数据展示

  • IHC staining of human stomach cancer using 82890-2-RR

    IHC staining of human stomach cancer using 82890-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82890-2-RR (RRBP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with RRBP1 antibody included
  • IF Staining of HepG2 using 82890-2-RR

    IF Staining of HepG2 using 82890-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRBP1 antibody (82890-2-RR, Clone: 230159G12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • FC experiment of A549 using 82890-2-RR

    FC experiment of A549 using 82890-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-2-RR, Clone:230159G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of U2OS using 82890-2-RR

    FC experiment of U2OS using 82890-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-2-RR, Clone:230159G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82890-2-RR

    Affinity and Kinetic Characterization of 82890-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-2-RR against Human RRBP1 were performed. The affinity constant is 1.29 nM.

RRBP1 Recombinant antibody

RRBP1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82890-2-RR

产品说明书

CloneNo. 230159G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G12, 180 kDa ribosome receptor homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82890-2-RR targets RRBP1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RRBP1 fusion protein Ag16648 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosome binding protein 1 homolog 180kDa (dog)
别名 Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G12, 180 kDa ribosome receptor homolog
计算分子量 1410 aa, 152 kDa
观测分子量108 kDa, 152 kDa
GenBank蛋白编号BC128577
基因名称 RRBP1
Gene ID (NCBI) 6238
RRIDAB_3670615
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2E9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

实验方案

Product Specific Protocols
IHC protocol for RRBP1 antibody 82890-2-RRDownload protocol
IF protocol for RRBP1 antibody 82890-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols