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验证数据展示

  • WB analysis using 27706-1-AP

    WB analysis using 27706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27706-1-AP (RREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27706-1-AP

    IHC staining of human colon cancer using 27706-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 27706-1-AP

    IHC staining of human ovary cancer using 27706-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RREB1 Polyclonal antibody

RREB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27706-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells
Positive IHC detected inhuman colon cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27706-1-AP targets RREB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RREB1 fusion protein Ag26780 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ras responsive element binding protein 1
别名 RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT
计算分子量 181 kDa
观测分子量 250 kDa
GenBank蛋白编号NM_001003698
基因名称 RREB1
Gene ID (NCBI) 6239
RRIDAB_2880948
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family.RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. RREB1 may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

实验方案

Product Specific Protocols
WB protocol for RREB1 antibody 27706-1-APDownload protocol
IHC protocol for RREB1 antibody 27706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols