验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, SH-SY5Y cells, HeLa cells |
| Positive IHC detected in | human stomach tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20280-1-AP targets RREB1-Specific in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ras responsive element binding protein 1 |
| 别名 | RREB1, RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321 |
| 计算分子量 | 181 kDa |
| 观测分子量 | 160-181 kDa, 250 kDa |
| GenBank蛋白编号 | NM_001003698 |
| 基因名称 | RREB1 |
| Gene ID (NCBI) | 6239 |
| RRID | AB_2878661 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92766 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RREB1-Specific antibody 20280-1-AP | Download protocol |
| IHC protocol for RREB1-Specific antibody 20280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cycle Knockdown of RREB1 inhibits cell proliferation via enhanced p16 expression in gastric cancer.
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Cell Rep Oncogenic KRAS effector USP13 promotes metastasis in non-small cell lung cancer through deubiquitinating β-catenin
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