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RREB1-Specific Polyclonal antibody
RREB1-Specific Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, SH-SY5Y cells, HeLa cells |
| Positive IHC detected in | human stomach tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20280-1-AP targets RREB1-Specific in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, ChIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ras responsive element binding protein 1 |
| Synonyms | RREB1, RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321 |
| Calculated Molecular Weight | 181 kDa |
| Observed Molecular Weight | 160-181 kDa, 250 kDa |
| GenBank Accession Number | NM_001003698 |
| Gene Symbol | RREB1 |
| Gene ID (NCBI) | 6239 |
| RRID | AB_2878661 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RREB1-Specific antibody 20280-1-AP | Download protocol |
| IHC protocol for RREB1-Specific antibody 20280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cycle Knockdown of RREB1 inhibits cell proliferation via enhanced p16 expression in gastric cancer.
| ||
Cell Rep Oncogenic KRAS effector USP13 promotes metastasis in non-small cell lung cancer through deubiquitinating β-catenin
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