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验证数据展示

  • WB analysis using 84048-1-RR (same clone as 84048-1-PBS)

    WB analysis using 84048-1-RR (same clone as 84048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84048-1-RR (RREB1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84048-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84048-1-RR (same clone as 84048-1-PBS)

    IF Staining of HeLa using 84048-1-RR (same clone as 84048-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RREB1-Specific antibody (84048-1-RR, Clone: 240800B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84048-1-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 84048-1-RR (same clone as 84048-1-PBS)

    FC experiment of U-2 OS using 84048-1-RR (same clone as 84048-1-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug RREB1-Specific Recombinant antibody (84048-1-RR, Clone:240800B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84048-1-PBS in a different storage buffer formulation.

View 3D epitope

RREB1-Specific Recombinant antibody, PBS Only

RREB1-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84048-1-PBS

产品说明书

CloneNo. 240800B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

RREB1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 
100 μg

¥5800/ 100 μg

浓度: 1 mg/ml

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产品信息

84048-1-PBS targets RREB1-Specific in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ras responsive element binding protein 1
别名 RREB1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT
计算分子量 181 kDa
观测分子量260 kDa
GenBank蛋白编号NM_001003698
基因名称 RREB1
Gene ID (NCBI) 6239
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92766
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)