RRM2 Polyclonal antibody
RRM2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A431 cells, HUVEC cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11661-1-AP targets RRM2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse, chicken |
Immunogen | RRM2 fusion protein Ag2203 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribonucleotide reductase M2 polypeptide |
Synonyms | RR2M, RR2, R2, EC:1.17.4.1 |
Calculated Molecular Weight | 389 aa, 45 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC030154 |
Gene Symbol | RRM2 |
Gene ID (NCBI) | 6241 |
RRID | AB_2180392 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P31350 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RRM2 antibody 11661-1-AP | Download protocol |
IHC protocol for RRM2 antibody 11661-1-AP | Download protocol |
IF protocol for RRM2 antibody 11661-1-AP | Download protocol |
IP protocol for RRM2 antibody 11661-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis | ||
Oxid Med Cell Longev lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients. | ||
Front Cell Dev Biol A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer. | ||
Antiviral Res Long-term HIV-1 infection induces an antiviral state in primary macrophages. | ||
Front Oncol Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes. | ||
Biomed Pharmacother Identification of osalmid metabolic profile and active metabolites with anti-tumor activity in human hepatocellular carcinoma cells. |