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RRM2 Monoclonal antibody, PBS Only

RRM2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2A9A7

Cat no : 67006-1-PBS

Print datasheet

Synonyms

RR2M, RR2, R2, EC:1.17.4.1, 2A9A7

验证数据展示

  • WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

    WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

    IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

    IP result of anti-RRM2 (IP:67006-1-Ig, 4ug; Detection:67006-1-Ig 1:2000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67006-1-Ig (same clone as 67006-1-PBS)

    IHC staining of human skin cancer using 67006-1-Ig (same clone as 67006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67006-1-Ig (RRM2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRM2 antibody included
  • IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

    IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM2 antibody (67006-1-Ig, Clone: 2A9A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RRM2 Ready-To-Use IHC Kit. RRM2 primary antibody included.

产品信息

67006-1-PBS targets RRM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen RRM2 fusion protein Ag28664 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribonucleotide reductase M2 polypeptide
Synonyms RR2M, RR2, R2, EC:1.17.4.1, 2A9A7
Calculated Molecular Weight 389 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC030154
Gene Symbol RRM2
Gene ID (NCBI) 6241
RRIDAB_2882323
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31350
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].