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RRM2 Monoclonal antibody, PBS Only

RRM2 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2A9A7

Cat No : 67006-1-PBS

Print datasheet

Synonyms

R2, RR2, RR2M, RRM2

验证数据展示

  • WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

    WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

    IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67006-1-Ig (RRM2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

    IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

    IP result of anti-RRM2 (IP:67006-1-Ig, 4ug; Detection:67006-1-Ig 1:2000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

产品信息

67006-1-PBS targets RRM2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human
Immunogen RRM2 fusion protein Ag28664 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribonucleotide reductase M2 polypeptide
Synonyms R2, RR2, RR2M, RRM2
Calculated Molecular Weight 389 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC030154
Gene Symbol RRM2
Gene ID (NCBI) 6241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31350
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].