CoraLite® Plus 488-conjugated RRM2 Monoclonal antibody
RRM2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A9A7
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RRM2 Ready-To-Use IHC Kit. RRM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67006-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67006 targets RRM2 in IF/ICC applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human |
| Immunogen | RRM2 fusion protein Ag28664 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ribonucleotide reductase M2 polypeptide |
| Synonyms | R2, RR2, RR2M, RRM2 |
| Calculated Molecular Weight | 389 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC030154 |
| Gene Symbol | RRM2 |
| Gene ID (NCBI) | 6241 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31350 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RRM2 antibody CL488-67006 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |