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  • Featured Product
  • KD/KO Validated

RRM2B,p53R2 Polyclonal antibody

RRM2B,p53R2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18005-1-AP

Print datasheet

Synonyms

RRM2B, TP53-inducible ribonucleotide reductase M2 B, Ribonucleoside-diphosphate reductase subunit M2 B, p53R2, p53-inducible ribonucleotide reductase small subunit 2-like protein

验证数据展示

  • WB analysis using 18005-1-AP

    WB analysis using 18005-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 18005-1-AP

    IHC staining of human stomach using 18005-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, PC-3 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18005-1-AP targets RRM2B,p53R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RRM2B,p53R2 fusion protein Ag12499 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonucleotide reductase M2 B (TP53 inducible)
Synonyms RRM2B, TP53-inducible ribonucleotide reductase M2 B, Ribonucleoside-diphosphate reductase subunit M2 B, p53R2, p53-inducible ribonucleotide reductase small subunit 2-like protein
Calculated Molecular Weight 351 aa, 41 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC108261
Gene Symbol RRM2B
Gene ID (NCBI) 50484
RRIDAB_2878480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LG56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
IHC protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
  • KD Validated
View Article