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验证数据展示

  • WB analysis using 18005-1-AP

    WB analysis using 18005-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 18005-1-AP

    IHC staining of human stomach using 18005-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

RRM2B,p53R2 Polyclonal antibody

RRM2B,p53R2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18005-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

RRM2B, TP53-inducible ribonucleotide reductase M2 B, Ribonucleoside-diphosphate reductase subunit M2 B, p53R2, p53-inducible ribonucleotide reductase small subunit 2-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, PC-3 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18005-1-AP targets RRM2B,p53R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RRM2B,p53R2 fusion protein Ag12499 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonucleotide reductase M2 B (TP53 inducible)
别名 RRM2B, TP53-inducible ribonucleotide reductase M2 B, Ribonucleoside-diphosphate reductase subunit M2 B, p53R2, p53-inducible ribonucleotide reductase small subunit 2-like protein
计算分子量 351 aa, 41 kDa
观测分子量 42 kDa
GenBank蛋白编号BC108261
基因名称 RRM2B
Gene ID (NCBI) 50484
RRIDAB_2878480
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7LG56
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
IHC protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
  • KD Validated
View Article