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RRN3 Polyclonal antibody

RRN3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25918-1-AP

Print datasheet

Synonyms

RRN3, TIF IA, TIFIA

验证数据展示

  • WB analysis of HeLa using 25918-1-AP

    WB analysis of HeLa using 25918-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25918-1-AP

    IP experiment of HeLa using 25918-1-AP

    IP result of anti-RRN3 (IP:25918-1-AP, 4ug; Detection:25918-1-AP 1:500) with HeLa cells lysate 3200ug.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, mouse testis tissue
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25918-1-AP targets RRN3 in WB, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, chIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RRN3 fusion protein Ag23207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Synonyms RRN3, TIF IA, TIFIA
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC132688
Gene Symbol RRN3
Gene ID (NCBI) 54700
RRIDAB_2880296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRN3 antibody 25918-1-APDownload protocol
IP protocol for RRN3 antibody 25918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

Biochem Biophys Res Commun

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Authors - Xiaolei Zhang
View Article
WB

Mol Cell

Control of ribosomal RNA synthesis by hematopoietic transcription factors

Authors - Charles Antony
View Article