• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RRP8 Polyclonal antibody

RRP8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20129-1-AP

Print datasheet

Synonyms

Nucleomethylin, NML, KIAA0409, hucep-1, EC:2.1.1.-

验证数据展示

  • WB analysis of human brain using 20129-1-AP

    WB analysis of human brain using 20129-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20129-1-AP (RRP8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20129-1-AP

    IHC staining of human skin using 20129-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20129-1-AP (RRP8 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 20129-1-AP

    IHC staining of human skin using 20129-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20129-1-AP (RRP8 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20129-1-AP

    IF Staining of HeLa using 20129-1-AP

    Immunofluorescent analysis of Hela cells, using RRP8 antibody 20129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20129-1-AP targets RRP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RRP8 fusion protein Ag13994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal RNA processing 8, methyltransferase, homolog (yeast)
Synonyms Nucleomethylin, NML, KIAA0409, hucep-1, EC:2.1.1.-
Calculated Molecular Weight 456 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC001071
Gene Symbol RRP8
Gene ID (NCBI) 23378
RRIDAB_10665422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRP8 antibody 20129-1-APDownload protocol
IHC protocol for RRP8 antibody 20129-1-APDownload protocol
IF protocol for RRP8 antibody 20129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cancer Res Clin Oncol

RRP8, associated with immune infiltration, is a prospective therapeutic target in hepatocellular carcinoma

Authors - Kai You
View Article