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  • Featured Product
  • KD/KO Validated

RSF1 Polyclonal antibody

RSF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26011-1-AP

Print datasheet

Synonyms

HBV pX associated protein 8, HBXAP, p325, RSF 1, RSF1, XAP8

验证数据展示

  • WB analysis using 26011-1-AP

    WB analysis using 26011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26011-1-AP (RSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 26011-1-AP

    WB analysis of HeLa using 26011-1-AP

    WB result of RSF1 antibody (26011-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSF1 transfected HeLa cells.

经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293 cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26011-1-AP targets RSF1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RSF1 fusion protein Ag22456 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name remodeling and spacing factor 1
Synonyms HBV pX associated protein 8, HBXAP, p325, RSF 1, RSF1, XAP8
Calculated Molecular Weight 1441 aa, 164 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC153210
Gene Symbol RSF1
Gene ID (NCBI) 51773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96T23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. The calculated molecular weight of RSF1 is 163 kDa, but 26011-1-AP can detect a band around 250 kDa, we validated the specificity by knock down. (PMID: 34147108)

实验方案

Product Specific Protocols
WB protocol for RSF1 antibody 26011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols