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验证数据展示

  • WB analysis using 26011-1-AP

    WB analysis using 26011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26011-1-AP (RSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 26011-1-AP

    WB analysis of HeLa using 26011-1-AP

    WB result of RSF1 antibody (26011-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSF1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

RSF1 Polyclonal antibody

RSF1 Polyclonal Antibody for WB, ELISA
Cat No. 26011-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HBV pX associated protein 8, HBXAP, p325, RSF 1, RSF1, XAP8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293 cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26011-1-AP targets RSF1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RSF1 fusion protein Ag22456 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 remodeling and spacing factor 1
别名 HBV pX associated protein 8, HBXAP, p325, RSF 1, RSF1, XAP8
计算分子量 1441 aa, 164 kDa
观测分子量250 kDa
GenBank蛋白编号BC153210
基因名称 RSF1
Gene ID (NCBI) 51773
RRIDAB_3085834
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96T23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. The calculated molecular weight of RSF1 is 163 kDa, but 26011-1-AP can detect a band around 250 kDa, we validated the specificity by knock down. (PMID: 34147108)

实验方案

Product Specific Protocols
WB protocol for RSF1 antibody 26011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols