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验证数据展示

  • WB analysis of HepG2 using 25190-1-AP

    WB analysis of HepG2 using 25190-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25190-1-AP

    IF Staining of HepG2 using 25190-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSL24D1 antibody (25190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RSL24D1 Polyclonal antibody

RSL24D1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25190-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

Ribosomal protein L24-like, Ribosomal L24 domain-containing protein 1, My024, L30, HRP L30 iso

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HL-60 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25190-1-AP targets RSL24D1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 RSL24D1 fusion protein Ag18195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal L24 domain containing 1
别名 Ribosomal protein L24-like, Ribosomal L24 domain-containing protein 1, My024, L30, HRP L30 iso
计算分子量 163 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC008409
基因名称 RSL24D1
Gene ID (NCBI) 51187
RRIDAB_2879950
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHA3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RSL24D1 antibody 25190-1-APDownload protocol
IF protocol for RSL24D1 antibody 25190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.

Authors - Chunyang Ni
View Article
humanWB

RNA

Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing

Authors - Mason A McCool
View Article
humanIF

mBio

Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication

Authors - Maikke B Ohlson
View Article
humanWB

Cell

The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity

Authors - Vidhya Krishnamoorthy
View Article