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RSPO3 Monoclonal antibody

RSPO3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E4F1

Cat no : 66314-1-Ig

Print datasheet

Synonyms

Protein with TSP type-1 repeat, Protein with TSP type 1 repeat, hRspo3, hPWTSR, CRISTIN1

验证数据展示

  • WB analysis of human skeletal muscle using 66314-1-Ig

    WB analysis of human skeletal muscle using 66314-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66314-1-Ig

    IHC staining of human lung cancer using 66314-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 66314-1-Ig

    IHC staining of human lung cancer using 66314-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 66314-1-Ig

    IF Staining of A549 using 66314-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RSPO3 antibody (66314-1-Ig, Clone: 1E4F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, A549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66314-1-Ig targets RSPO3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen RSPO3 fusion protein Ag9899 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name R-spondin 3 homolog (Xenopus laevis)
Synonyms Protein with TSP type-1 repeat, Protein with TSP type 1 repeat, hRspo3, hPWTSR, CRISTIN1
Calculated Molecular Weight 272 aa, 31 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC022367
Gene Symbol RSPO3
Gene ID (NCBI) 84870
RRIDAB_2881695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BXY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

实验方案

Product Specific Protocols
WB protocol for RSPO3 antibody 66314-1-IgDownload protocol
IHC protocol for RSPO3 antibody 66314-1-IgDownload protocol
IF protocol for RSPO3 antibody 66314-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols