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  • Featured Product
  • KD/KO Validated

RSRC1 Polyclonal antibody

RSRC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23826-1-AP

Print datasheet

Synonyms

SRrp53, Serine/Arginine-related protein 53, BM-011, BM 011, Arginine/serine-rich coiled-coil protein 1

验证数据展示

  • WB analysis of HeLa using 23826-1-AP

    WB analysis of HeLa using 23826-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23826-1-AP (RSRC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 23826-1-AP

    IHC staining of human small intestine using 23826-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 23826-1-AP

    IHC staining of human small intestine using 23826-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 23826-1-AP

    IF Staining of HepG2 using 23826-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSRC1 antibody (23826-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23826-1-AP targets RSRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen RSRC1 fusion protein Ag20604 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arginine/serine-rich coiled-coil 1
Synonyms SRrp53, Serine/Arginine-related protein 53, BM-011, BM 011, Arginine/serine-rich coiled-coil protein 1
Calculated Molecular Weight 334 aa, 39 kDa
Observed Molecular Weight 38 kDa, 50 kDa
GenBank Accession NumberBC006982
Gene Symbol RSRC1
Gene ID (NCBI) 51319
RRIDAB_2879329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96IZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RSRC1, also named as SRRP53, is a novel SR-related protein of an apparent molecular mass of 53 kDa (45-53 kDa). It is isolated in a gene trap screen that identifies proteins which localize to the nuclear speckles. RSRC1 plays a rol in pre-mRNA splicing and constitutive.

实验方案

Product Specific Protocols
WB protocol for RSRC1 antibody 23826-1-APDownload protocol
IHC protocol for RSRC1 antibody 23826-1-APDownload protocol
IF protocol for RSRC1 antibody 23826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Mol Med Rep

RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression.

Authors - Shijun Yu
  • KD Validated
View Article