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  • Featured Product
  • KD/KO Validated

RTCD1 Polyclonal antibody

RTCD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15996-1-AP

Print datasheet

Synonyms

RNA 3 phosphate cyclase, RNA cyclase, RPC, RPC1, RTC1, RTCD1

验证数据展示

  • WB analysis using 15996-1-AP

    WB analysis using 15996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15996-1-AP

    IHC staining of mouse testis using 15996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15996-1-AP

    IHC staining of mouse testis using 15996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, THP-1 cells, U-251 cells, U-87 MG cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15996-1-AP targets RTCD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RTCD1 fusion protein Ag8904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA terminal phosphate cyclase domain 1
Synonyms RNA 3 phosphate cyclase, RNA cyclase, RPC, RPC1, RTC1, RTCD1
Calculated Molecular Weight 379 aa, 41 kDa
Observed Molecular Weight 41-43 kDa
GenBank Accession NumberBC012604
Gene Symbol RTCD1
Gene ID (NCBI) 8634
RRIDAB_2180923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RTCD1 antibody 15996-1-APDownload protocol
IHC protocol for RTCD1 antibody 15996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Authors - Irem Unlu
  • KD Validated
View Article