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RUFY2 Polyclonal antibody

RUFY2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19180-1-AP

Print datasheet

Synonyms

KIAA1537, RABIP4R, RUFY2, ZFYVE13

验证数据展示

  • WB analysis of mouse lung using 19180-1-AP

    WB analysis of mouse lung using 19180-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19180-1-AP

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 19180-1-AP

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse ovary tissue, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19180-1-AP targets RUFY2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RUFY2 fusion protein Ag5938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RUN and FYVE domain containing 2
Synonyms KIAA1537, RABIP4R, RUFY2, ZFYVE13
Calculated Molecular Weight 403 aa, 46 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC041092
Gene Symbol RUFY2
Gene ID (NCBI) 55680
RRIDAB_10643382
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXA3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RUFY2 antibody 19180-1-APDownload protocol
IHC protocol for RUFY2 antibody 19180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols