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  • Featured Product
  • KD/KO Validated

RUNDC3A Polyclonal antibody

RUNDC3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20531-1-AP

Print datasheet

Synonyms

Rap2 interacting protein 8, RAP2IP, RPIP 8, RPIP8, RUN domain containing 3A, RUNDC3A

验证数据展示

  • WB analysis using 20531-1-AP

    WB analysis using 20531-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20531-1-AP (RUNDC3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20531-1-AP

    IHC staining of mouse brain using 20531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20531-1-AP (RUNDC3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20531-1-AP

    IHC staining of mouse brain using 20531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20531-1-AP (RUNDC3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-12 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20531-1-AP targets RUNDC3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RUNDC3A fusion protein Ag14393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RUN domain containing 3A
Synonyms Rap2 interacting protein 8, RAP2IP, RPIP 8, RPIP8, RUN domain containing 3A, RUNDC3A
Calculated Molecular Weight 446 aa, 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC006194
Gene Symbol RUNDC3A
Gene ID (NCBI) 10900
RRIDAB_10695757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ59EK9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RUNDC3A antibody 20531-1-APDownload protocol
IHC protocol for RUNDC3A antibody 20531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Discov

RUNDC3A regulates SNAP25-mediated chemotherapy resistance by binding AKT in gastric neuroendocrine carcinoma (GNEC).

Authors - Pengchen Chen
  • KO Validated
View Article