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验证数据展示

  • WB analysis of MCF-7 using 17113-1-AP

    WB analysis of MCF-7 using 17113-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17113-1-AP (RUNDC3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 17113-1-AP

    IF Staining of MCF-7 using 17113-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17113-1-AP (RUNDC3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RUNDC3B Polyclonal antibody

RUNDC3B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17113-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RPIP 9, RPIB9, Rap2-interacting protein 9, Rap2-binding protein 9, Rap2 interacting protein 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, mouse brain tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17113-1-AP targets RUNDC3B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RUNDC3B fusion protein Ag10797 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RUN domain containing 3B
别名 RPIP 9, RPIB9, Rap2-interacting protein 9, Rap2-binding protein 9, Rap2 interacting protein 9
计算分子量 387 aa, 43 kDa
观测分子量 45 kDa
GenBank蛋白编号BC022520
基因名称 RUNDC3B
Gene ID (NCBI) 154661
RRIDAB_10699872
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NL0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RUNDC3B antibody 17113-1-APDownload protocol
IF protocol for RUNDC3B antibody 17113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols