RUNX1 (N-terminal) Polyclonal antibody

RUNX1 (N-terminal) Polyclonal Antibody for WB, IP, ELISA
Cat No. 19555-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, ELISA and More (2)

RUNX1, AML1, AML1 EVI 1, AMLCR1, CBF alpha 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19555-1-AP targets RUNX1 (N-terminal) in WB, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, CoIP, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 runt-related transcription factor 1
别名 RUNX1, AML1, AML1 EVI 1, AMLCR1, CBF alpha 2
计算分子量 51 kDa
观测分子量 48-55 kDa
GenBank蛋白编号NM_001754
基因名称 RUNX1
Gene ID (NCBI) 861
RRIDAB_10949190
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01196
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11.

实验方案

Product Specific Protocols
WB protocol for RUNX1 (N-terminal) antibody 19555-1-APDownload protocol
IP protocol for RUNX1 (N-terminal) antibody 19555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Protein Expr Purif

Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.

Authors - Guoqiang Zhou
humanWB,CoIP

Mol Cells

RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15

Authors - Hao Ding
ratChIP,WB

Respir Res

RUNX1 targeting AKT3 promotes alveolar hypercoagulation and fibrinolytic inhibition in LPS induced ARDS

Authors - Chuan Xiao
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