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验证数据展示

  • WB analysis using 10195-1-AP

    WB analysis using 10195-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10195-1-AP (RUVBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa cells using 10195-1-AP

    IP experiment of HeLa cells using 10195-1-AP

    IP result of anti-RUVBL2 (10195-1-AP for IP and Detection).

  • IF Staining of HepG2 using 10195-1-AP

    IF Staining of HepG2 using 10195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RUVBL2 antibody (10195-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human placenta using 10195-1-AP

    IHC staining of human placenta using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of human placenta using 10195-1-AP

    IHC staining of human placenta using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of mouse bladder using 10195-1-AP

    IHC staining of mouse bladder using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of mouse bladder using 10195-1-AP

    IHC staining of mouse bladder using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of rat bladder using 10195-1-AP

    IHC staining of rat bladder using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of rat bladder using 10195-1-AP

    IHC staining of rat bladder using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of human gliomas using 10195-1-AP

    IHC staining of human gliomas using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IHC staining of human gliomas using 10195-1-AP

    IHC staining of human gliomas using 10195-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • Featured Product
  • KD/KO验证

RUVBL2 Polyclonal antibody

RUVBL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10195-1-AP

产品说明书

引用文献(22)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

RUVB2, INO80J, ECP-51, EC:3.6.4.12, CGI-46

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells, Daudi cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inrat bladder tissue, human gliomas tissue, human placenta tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RUVBL2 Ready-To-Use IHC Kit. RUVBL2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10195-1-AP targets RUVBL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 RUVBL2 fusion protein Ag0253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RuvB-like 2 (E. coli)
别名 RUVB2, INO80J, ECP-51, EC:3.6.4.12, CGI-46
计算分子量 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC000428
基因名称 RUVBL2
Gene ID (NCBI) 10856
RRIDAB_2184679
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y230
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.

实验方案

Product Specific Protocols
WB protocol for RUVBL2 antibody 10195-1-APDownload protocol
IHC protocol for RUVBL2 antibody 10195-1-APDownload protocol
IF protocol for RUVBL2 antibody 10195-1-APDownload protocol
IP protocol for RUVBL2 antibody 10195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Authors - Sang Gyun Kim
View Article
humanWB,IF

Sci Adv

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Authors - Seung Ho Shin
  • KD Validated
View Article
humanWB,IP

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
View Article
mouseIP

J Clin Invest

circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.

Authors - Zhenzhen Chen
View Article
humanWB

Sci Adv

ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.

Authors - Franklin C Harwood
View Article
mouseWB

EMBO J

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang
View Article