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验证数据展示

  • WB analysis of K-562 using 11365-1-AP

    WB analysis of K-562 using 11365-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11365-1-AP (RYBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 11365-1-AP

    IP experiment of mouse spleen using 11365-1-AP

    IP result of anti-RYBP (IP:11365-1-AP, 4ug; Detection:11365-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

RYBP Polyclonal antibody

RYBP Polyclonal Antibody for WB, IP, ELISA
Cat No. 11365-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11365-1-AP targets RYBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RYBP fusion protein Ag1908 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RING1 and YY1 binding protein
别名 AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1
计算分子量 228 aa, 25 kDa
观测分子量 32 kDa
GenBank蛋白编号BC014959
基因名称 RYBP
Gene ID (NCBI) 23429
RRIDAB_2285461
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N488
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

实验方案

Product Specific Protocols
WB protocol for RYBP antibody 11365-1-APDownload protocol
IP protocol for RYBP antibody 11365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
View Article