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RYBP Polyclonal antibody

RYBP Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11365-1-AP

Print datasheet

Synonyms

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

验证数据展示

  • WB analysis of K-562 using 11365-1-AP

    WB analysis of K-562 using 11365-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11365-1-AP (RYBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 11365-1-AP

    IP experiment of mouse spleen using 11365-1-AP

    IP result of anti-RYBP (IP:11365-1-AP, 4ug; Detection:11365-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inmouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11365-1-AP targets RYBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RYBP fusion protein Ag1908 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RING1 and YY1 binding protein
Synonyms AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC014959
Gene Symbol RYBP
Gene ID (NCBI) 23429
RRIDAB_2285461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

实验方案

Product Specific Protocols
WB protocol for RYBP antibody 11365-1-APDownload protocol
IP protocol for RYBP antibody 11365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
View Article