验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, A549 cells, mouse brain tissue, SH-SY5Y cells |
Positive IHC detected in | human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
产品信息
22138-1-AP targets RYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RYK fusion protein Ag17540 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RYK receptor-like tyrosine kinase |
别名 | Tyrosine protein kinase RYK, RYK1, JTK5A, JTK5, EC:2.7.10.1 |
计算分子量 | 610 aa, 68 kDa |
观测分子量 | 67-75 kDa |
GenBank蛋白编号 | BC153090 |
基因名称 | RYK |
Gene ID (NCBI) | 6259 |
RRID | AB_11232602 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P34925 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tyrosine-protein kinase RYK may be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A, and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment, and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region, allowing the C-terminal intracellular product to translocate from the cytoplasm to the nucleus, which plays a crucial role in neuronal development.
实验方案
Product Specific Protocols | |
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WB protocol for RYK antibody 22138-1-AP | Download protocol |
IHC protocol for RYK antibody 22138-1-AP | Download protocol |
IF protocol for RYK antibody 22138-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Neuroinflammation Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model | ||
Cell Mol Biol Lett Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway | ||
Pharmaceuticals (Basel) RYK Gene Expression Associated with Drug Response Variation of Temozolomide and Clinical Outcomes in Glioma Patients |