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RYR1 Polyclonal antibody

RYR1 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26968-1-AP

Print datasheet

Synonyms

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, RYR1/2/3, SKRR

验证数据展示

  • IHC staining of mouse brain using 26968-1-AP

    IHC staining of mouse brain using 26968-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26968-1-AP

    IHC staining of mouse brain using 26968-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 26968-1-AP

    IF Staining of mouse cerebellum using 26968-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RYR1 antibody (26968-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab.

  • IF Staining of mouse brain using 26968-1-AP

    IF Staining of mouse brain using 26968-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RYR1 antibody (26968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue, human cerebellum tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue, mouse brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

26968-1-AP targets RYR1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen RYR1 fusion protein Ag25120 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ryanodine receptor 1 (skeletal)
Synonyms CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, RYR1/2/3, SKRR
Calculated Molecular Weight 565 kDa
GenBank Accession NumberNM_000540
Gene Symbol RYR1
Gene ID (NCBI) 6261
RRIDAB_2880703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.

实验方案

Product Specific Protocols
IHC protocol for RYR1 antibody 26968-1-APDownload protocol
IF protocol for RYR1 antibody 26968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

JCI Insight

Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism

Authors - Benedict Abdon
View Article