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验证数据展示

  • IHC staining of mouse brain using 26968-1-AP

    IHC staining of mouse brain using 26968-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26968-1-AP

    IHC staining of mouse brain using 26968-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 26968-1-AP

    IF Staining of mouse cerebellum using 26968-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RYR1 antibody (26968-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab.

  • IF Staining of mouse brain using 26968-1-AP

    IF Staining of mouse brain using 26968-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RYR1 antibody (26968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

RYR1 Polyclonal antibody

RYR1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 26968-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

IHC, IF-P, ELISA and More (2)

别名

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, RYR1/2/3, SKRR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, human cerebellum tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue, mouse brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26968-1-AP targets RYR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse, chicken
免疫原 RYR1 fusion protein Ag25120 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ryanodine receptor 1 (skeletal)
别名 CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, RYR1/2/3, SKRR
计算分子量 565 kDa
GenBank蛋白编号NM_000540
基因名称 RYR1
Gene ID (NCBI) 6261
RRIDAB_2880703
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21817
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.

实验方案

Product Specific Protocols
IHC protocol for RYR1 antibody 26968-1-APDownload protocol
IF protocol for RYR1 antibody 26968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

JCI Insight

Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism

Authors - Benedict Abdon
View Article
chickenWB

Poult Sci

Effects of chronic heat stress on Ca2+ homeostasis, apoptosis, and protein carbonylation profiles in the breast muscle of broilers

Authors - Yingsen Liu
View Article