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验证数据展示

  • IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 66539-1-Ig (same clone as 66539-1-PBS)

    IHC staining of mouse cerebellum using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • IF Staining of A-673 using 66539-1-Ig (same clone as 66539-1-PBS)

    IF Staining of A-673 using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A-673 cells using RYR1 antibody (66539-1-Ig, Clone: 1B4E12 ) at dilution of 1:300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

RYR1 Monoclonal antibody, PBS Only

RYR1 Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 66539-1-PBS

产品说明书

CloneNo. 1B4E12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

SKRR, RYR1/2/3, RYDR, Ryanodine receptor 1, CCO

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66539-1-PBS targets RYR1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RYR1 fusion protein Ag25120 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ryanodine receptor 1 (skeletal)
别名 SKRR, RYR1/2/3, RYDR, Ryanodine receptor 1, CCO
计算分子量 565 kDa
GenBank蛋白编号NM_000540
基因名称 RYR1
Gene ID (NCBI) 6261
RRIDAB_2881901
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21817
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.