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Recombinant Rat IFN-gamma protein (His Tag)

ED50

6-22 ng/mL

Species

Rat

Purity

>95 %, SDS-PAGE

GeneID

25712

Accession

P01581

Cat No : Eg0400

Print datasheet

Synonyms

IFNG, IFN-gamma, IFN-γ, Interferon gamma

验证数据展示

  • Purity of Recombinant Rat IFN-gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat IFN-gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Rat IFN-gamma (His tag) at 1 μg/mL (100 μL/well) can bind Rat IFN-gamma R1 (C-hFc tag) with a linear range of 6-22 ng/mL.

    Immobilized Rat IFN-gamma (His tag) at 1 μg/mL (100 μL/well) can bind Rat IFN-gamma R1 (C-hFc tag) with a linear range of 6-22 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Rat IFN-gamma (His tag) at 1 μg/mL (100 μL/well) can bind Rat IFN-gamma R1 (hFc tag) with a linear range of 6-22 ng/mL.
Source HEK293-derived Rat IFN-gamma protein Gln23-Cys156 (Accession# P01581) with a His tag at the C-terminus.
Predicted Molecular Mass 18 kDa
SDS-PAGE 22-32 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The IFNs were originally discovered as agents that interfere with viral replication. Initially, they were classified by the secreting cell type but are now classified into type I and type II according to receptor specificity and sequence homology. Interferon gamma (Ifng) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant Ifng expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

References:

1.  Isaacs, A. et al.(1957) The interferon Proc. R. Soc. Lond. B Biol. Sci. 147: 258-267.
2.  Gray PW. et al. (1982) Nature. 298 : 859-63.
3.  Bullens, D. M. et al.(2001) Int. Immunol. 13: 181-191.
4.  Wang Z. et al.(2014) Biosci Biotechnol Biochem.78: 588-92.
5.  Chegou NN. et al.(2012)PLoS One.7(6):e38501
6.  Torok-Storb B. et al. (Blood) 69:629-33.