Rat IL-1 beta ELISA Kit

Sensitivity

1.9 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Rat

Cat No : KE20021

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Synonyms

Il1b, interleukin 1 beta



Product Information

KE20021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL-1 beta. The assay recognizes rat IL-1 beta. An antibody specific for rat IL-1 beta has been pre-coated onto the microwells. The rat IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for rat IL-1 beta is added to detect the captured rat IL-1 beta protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Rat IL-1 beta ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant
Assay type Sandwich
Sensitivity 1.9 pg/mL
Range 15.6-1000 pg/mL
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 24494

Recovery

Sample TypeAverageRange
Rat Serum114%102%-127%
Cell culture supernatant97%70%-128%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120470.720.44.3
220112.84.23.7
32028.81.24.1

InterAssay

Samplenmean ( pg/mL)SDCV%
124493.838.37.8
224110.05.44.9
32427.11.55.6

Background Information

Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il1b, interleukin 1 beta

Publications

SpeciesSample TypeTitle
ratcell culture supernatants

Int Immunopharmacol

Bardoxolone methyl breaks the vicious cycle between M1 macrophages and senescent nucleus pulposus cells through the Nrf2/STING/NF-κB pathway

Authors - Peng Wang
ratplasma

Biochim Biophys Acta Mol Basis Dis

Hyperglycemia and circadian disruption lead to retinal dysfunction in a stabilized colony of the fat sand rat Psammomys obesus

Authors - Catherine E Estay-Ahumada
human,ratcell lysates

Aging (Albany NY)

Medium-chain chlorinated paraffins (MCCPs) induce renal cell aging and ferroptosis

Authors - Dong Zhang
ratplasma

J Ethnopharmacol

Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway

Authors - Kai Zhang
rattissue homogenates

Front Pharmacol

Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats

Authors - Meiling Zhang
rat

Mol Neurobiol

Areca Thirteen Pill Improves Depression in Rat by Modulation of the Chemokine/Chemokine Receptor Axis

Authors - Jing-Jing Bai