Rat IL-10 ELISA Kit

Sensitivity

6.0 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Rat

Cat No : KE20022

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Synonyms

Il10, IL10X, interleukin 10



Product Information

KE20022 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL-10 ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL-10. The assay recognizes rat IL-10. An antibody specific for rat IL-10 has been pre-coated onto the microwells. The rat IL-10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat IL-10 is added to detect the captured rat IL-10 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Rat IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants,Tissue lysates,Tissue homogenizations
Assay type Sandwich
Sensitivity 6.0 pg/mL
Range 31.25-2000 pg/mL
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 25325

Recovery

Sample TypeAverageRange
Rat serum82%76-104%
Cell culture supernatants105%82%-119%
Tissue lysate86%76%-94%
Tissue homogenization101%83%-120%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,893.533.41.8
220461.910.52.3
320116.02.72.3

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,792.272.94.1
224435.49.22.1
324113.13.73.2

Background Information

IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il10, IL10X, interleukin 10