Rat IL-6 ELISA Kit
Validation Data Gallery
Product Information
KE20024 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL-6 ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL-6. The assay recognizes rat IL-6. An antibody specific for rat IL-6 has been pre-coated onto the microwells. The rat IL-6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat IL-6 is added to detect the captured rat IL-6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 29.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24498 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 86% | 77%-97% |
| Cell culture supernatant | 99% | 83%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,073.4 | 66.7 | 3.2 |
| 2 | 20 | 505.4 | 14.7 | 2.9 |
| 3 | 20 | 133.8 | 9.5 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,168.9 | 72.2 | 3.3 |
| 2 | 24 | 539.3 | 16.4 | 3.0 |
| 3 | 24 | 136.0 | 13.3 | 9.8 |
Background Information
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ifnb2, Il6, ILg6, interleukin 6 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Sci (Weinh) H2 S-Releasing Versatile Montmorillonite Nanoformulation Trilogically Renovates the Gut Microenvironment for Inflammatory Bowel Disease Modulation | ||
J Inflamm Res Curcumin Alleviates Osteoarthritis Through the p38MAPK Pathway: Network Pharmacological Prediction and Experimental Confirmation |