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Recombinant Rat IL-6 protein (His Tag)

ED50

50-200 ng/mL

Species

Rat

Purity

>95 %, SDS-PAGE

GeneID

24498

Accession

P20607

Cat No : Eg0800

Print datasheet

Synonyms

BSF2, CDF, HSF, IFNB2, IL6, IL-6, Interleukin-6

验证数据展示

  • Purity of Recombinant Rat IL-6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat IL-6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Rat IL-6 (His tag) at 2 μg/mL (100 μL/well) can bind Rat IL-6R alpha (hFc tag) with a linear range of 50-200 ng/mL.

    Immobilized Rat IL-6 (His tag) at 2 μg/mL (100 μL/well) can bind Rat IL-6R alpha (hFc tag) with a linear range of 50-200 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Rat IL-6 (His tag) at 2 μg/mL (100 μL/well) can bind Rat IL-6R alpha (hFc tag) with a linear range of 50-200 ng/mL.
Source HEK293-derived Rat IL-6 protein Phe25-Thr211 (Accession# P20607) with a His tag at the C-terminus.
Predicted Molecular Mass 22.5 kDa
SDS-PAGE 22-26 kDa
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

References:

1. Scheller J. et al. (1988). Behring Inst Mitt. Behring Inst Mitt. (83):40-7.
2. Heinrich PC. et al. (2003). Biochem J. 374: 1-20.
3. Rose-John S. et al. (2007). Expert Opin Ther Targets. 11: 613-24.
4. Ming JE. et al. (1989). J Mol Cell Immunol. 4: 203-11.
5. Febbraio MA. et al. (2005). Exerc Sport Sci Rev. 33:114-9.
6. J Appl Physiol. et al. (2005). J Appl Physiol. 98:1154-62.