Recombinant ALKBH1 protein

Recombinant ALKBH1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP.

ALKBH1 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH1 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.

Full length ALKBH1 protein (accession number NP_006011.2) was expressed in E.coli cells with an N-terminal 6xHis Tag. The molecular weight of ALKBH1 is 47.6 kDa.

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.