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Recombinant EHMT1 (894-1298) protein

Expressed In

E. coli

Protein Species

Human

Cat No : 31520,31920 31520

验证数据展示

  • Recombinant EHMT1 (894-1298) SDS-PAGE gel. Recombinant EHMT1 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 79.1 kDa Purity: > 85%

    Recombinant EHMT1 (894-1298) SDS-PAGE gel. Recombinant EHMT1 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 79.1 kDa Purity: > 85%

  • HTRF assay for EHMT1 (894-1298) activity 3 uM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.

    HTRF assay for EHMT1 (894-1298) activity 3 uM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.

  • HTRF assay for EHMT1 (894-1298) activity 3 uM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.

    HTRF assay for EHMT1 (894-1298) activity 3 uM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.

产品信息

Expressed In E. coli
Protein Species Human

Contents

Recombinant EHMT1 (894-1298) protein is supplied at a concentration of 0.8 ug/ul in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5% glycerol.

背景介绍

EHMT1(Euchromatic Histone Methyltransferase 1, EuHMT1, EuHMTase1, G9a like protein, GLP1) is a SET domain-containing histone methyltransferase specific for lysine 9 of histone H3. It is possible that this protein is involved in the silencing of Myc and E2F-responsive genes and thus could play a role in regulating cell cycle transitions from G0 to G1.

Application Notes

Recombinant EHMT1 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 3 uM H3 (1-21) peptide was incubated with different concentrations of EHMT1 (894-1298) protein in 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Protein Details

Recombinant EHMT1 (894-1298) protein that includes amino acids 894-1298 of human EHMT1 protein (accession number NP_079033.4) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 79.1 kDa. The purity of the protein is > 85% by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.