Recombinant SARS-CoV-2 NSP12 / RdRp protein

Recombinant SARS-CoV-2 NSP12 / RdRp protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.

Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). Coronaviruses are enveloped positive-stranded RNA viruses with the largest currently known RNA genomes. Expression of their genomes begins with the translation of two large replicase polyproteins, pp1a (>4,000 residues) and pp1ab (>7,000 residues), which are encoded by the viral replicase gene that comprises open reading frame 1a (orf1a) and orf1b1. pp1a and pp1ab are extensively processed by orf1a-encoded proteases to yield 15 or 16 mature nonstructural (replicase) proteins that assemble to form the membrane-associated viral replication and transcription machinery, which is vital to the viral life cycle. The nsp12, which is the first protein encoded by ORF1b, has common viral RNA-dependent RNA polymerase (RdRp) motifs and is thus the catalytic core of the RNA replicase.

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.

Recombinant SARS-CoV-2 NSP12 / RdRp protein was expressed in baculovirus expression system as the full length protein (accession number YP_009724389.1) with a N-terminal FLAG tag. The molecular weight of the protein is 107.9 kDa.

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.